Fig. 8.

ETV1-induced inflammatory expression signature precedes tumorigenesis. a Genes that are differentially expressed between hAR;Pten ^+/− and hAR;ETV1 ^Tg ;Pten ^+/− mice and annotated with inflammation-related GO terms (see Fig. 7a) are shown in the heatmap. Relative expression in prostates of hAR;Pten ^+/− and hAR;ETV1 ^Tg;Pten ^+/− mice is shown, as well as expression in the macroscopic prostate tumors. b RWPE-1 cells stably expressing AR and either ETV1 or LACZ were stimulated with 1 nM R1881 or methanol vehicle, and RNA was prepared for qRT-PCR analysis. Expression of the inflammatory genes CXCL16 and NUPR1 were normalized to GAPDH or ACTB, respectively, and plotted relative to RWPE-AR-LACZ cells. Mean and SEM are plotted for biological triplicate samples