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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Alcohol. 2015 Feb 7;49(3):219–227. doi: 10.1016/j.alcohol.2015.01.008

Figure 3.

Figure 3

Effects of exposure to 50 mM ethanol for 5 DIV, followed by one 24-hour period of ethanol withdrawal, and repeated for 1, 2, and 3 cycles of CIE, or exposure to continuous ethanol (50 mM) on thionine staining observed in organotypic slice cultures. Within the pyramidal cell layers of the CA1 (A) and CA3 (B), and granule cell layer of the dentate gyrus (C), exposure to 1 or 2 cycles of CIE, or continuous ethanol exposure did not result in significant decreases of thionine staining as compared to control values; whereas exposure 3 cycles of CIE in slices resulted in significant decreases of thionine staining as compared to control values in these hippocampal subregions (Figure 3). ** p < 0.001 vs control. Thionine staining of Nissl bodies was significantly decreased in hippocampi exposed to 3 cycles of CIE compared to hippocampi exposed to control medium (D).