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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Alcohol. 2015 Feb 7;49(3):219–227. doi: 10.1016/j.alcohol.2015.01.008

Figure 4.

Figure 4

Effects of exposure to 50 mM ethanol for 5 DIV, followed by one 24-hour period of ethanol withdrawal, and repeated for 1 or 2 CIE in aged slice cultures on NeuN immunoreactivity observed in organotypic slice cultures. Within the pyramidal cell layers of the CA1 and CA3, and granule cell layer of the dentate gyrus, exposure to 1 cycle of CIE in aged slice cultures did not result in significant decreases NeuN immunoreactivity (Figure 4) as compared to control values; whereas exposure 2 cycles of CIE in aged slices resulted in significant decreases of NeuN immunoreactivity compared to control values in these hippocampal subregions (Figure 4). *p <0.05 vs control.