Cytokines and cholinergic signaling genes were altered in placenta by maternal poly(I:C) injection and maternal choline supplementation. Maternal poly(I:C) injection increased Il6 mRNA in the placenta, and maternal choline supplementation did not prevent this induction. Maternal choline supplementation lowered Chrna7 and Ache mRNA in placenta after poly(I:C) injection. Gene expression was normalized by β-actin. Saline n = 3 litters; Poly(I:C) n = 4 litters, Poly(I:C)+Choline n = 3 litters. Ache: acetylcholinesterase, Chrna7: nicotinic acetylcholine receptor alpha 7 subunit, Il6: interleukin-6. Data are presented as Mean ± SEM. Significant difference between groups is labeled as * p < 0.05, ** p < 0.01.