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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: J Autoimmun. 2015 Feb 26;59:38–42. doi: 10.1016/j.jaut.2015.02.001

Figure 4. Generation of IL-10 producing cells by IL-2 stimulated APC.

Figure 4

Naïve NOD CD4+ T cells were cultured under neutral Th0 (A) or Th17 (B) conditions with T cell-depleted APC that were stimulated as indicated for 18h. On day 3 IL-10 in culture supernatant was determined by CBA (n=5). C) nTreg from NOD.FoxP3 KI mice were cultured with T cell-depleted syngeneic APC that were stimulated as indicated for 18h. Frequency of nTreg expressing T-bet and CXCR3 was determined by flow cytometry after 48h (n=3).