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. Author manuscript; available in PMC: 2015 Apr 30.
Published in final edited form as: ACS Chem Biol. 2015 Feb 4;10(4):970–977. doi: 10.1021/cb5009876

Figure 4.

Figure 4

Fluorescence polarization was monitored to determine the dissociation constants of the leader and core peptides to the synthetase. (A) HalM2 was titrated into fluorescein-labeled HalA2, (B) HalM2 was titrated into fluorescein-labeled HalA2 leader peptide, (C) HalM2 was titrated into 5-FAM labeled HalA2 core peptide, (D) HalCE2 was titrated into 5-FAM labeled HalA2 core peptide, and (E) HalM2 was titrated into 5-FAM labeled modified HalA2 peptide. All experiments were performed in at least triplicate and fitted to a hyperbolic curve and a dose-response curve in order to extrapolate the Kd. The error bars represent the standard deviation from the average of the triplicate measurements.