Fig. 3.

WISP-1 mediates the secretion of pro-angiogenic chemokines in fibroblasts. NRK-49F cells were stimulated with full-length WISP-1 prior to RNA extraction for gene microarray analysis. Data analysis using the GeneSpring GX11.0.2 software identified the enrichment of a subset of cytokines and chemokines (a). NRK-49F cells were stimulated with full-length (b) or truncated (d) WISP-1 and conditioned media assayed for CXCL3 content by ELISA. For inhibition studies, full-length WISP-1 was pre-incubated with anti-WISP-1 pAbs prior to addition to NRK-49F cells (c). Results for B-D are expressed as the mean ± SD (n = 3)