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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Nat Genet. 2015 Apr 6;47(5):528–534. doi: 10.1038/ng.3256

Figure 5. Morphant snx14 zebrafish show apoptosis, excessive autophagic vesicles, and loss of neural tissue including cerebellar primordium.

Figure 5

(a) Comparison of scrambled (6ng) and snx14 (3ng and 6ng) morphant zebrafish 48 hours postfertilization (hpf). Calipers: measured distance. Scale bar 250 µm. Graphs: Reduced optic tectum and right eye width in morphants. Mean ± SEM (n = 15 embryos for NI, 16 for Scramble, 31 for MO 3ng and 18 for MO 6ng, N = 2). *p < 0.05; **p < 0.005 (two tiled t-test). (b) Scramble or snx14 morphants for Zebrin II (Purkinje cell marker), rescued with human SNX14 (50 pg). Scale bar 50 µm. Graph: Zebrin II compartment area relative to scramble MO injected embryos. Mean ±SEM (n = 10 embryos for Scramble, 6 for MO 3ng, 9 for MO 6ng and 9 for rescue) *p < 0.05; **p < 0.005 (two tiled t-test). (c) Maximum confocal projection from 36 hpf Tg(ptf1a:eGFP) (green) zebrafish with scramble or snx14 MO showing reduced Purkinje cell progenitors. (d) Maximum confocal projection with increased caspase 3 (red) positive cells in 36 hpf snx14 morphants. Blue: DAPI. Scale bar 50 µm. (e) Transmission electron microscopy showing autophagic structures in 48 hpf snx14 and scrambled morphant neurons residing between the optic lobes. Box: Highlighted areas. Arrowheads: autophagic structures.