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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: J Cell Physiol. 2015 Aug;230(8):1929–1943. doi: 10.1002/jcp.24927

Figure 4. Sodium acetate is less effective at growth inhibition than GTA.

Figure 4

Free-floating GSCs in SCM (A), and adherent GSCs in DM (B) were treated with 0.25% triglycerides (canola oil), 0.25% glycerol, 12 mM sodium acetate (NaAc), 36 mM NaAc (equivalent acetate to 0.25% GTA), 36 mM NaAc plus 0.25% glycerol (equivalent to complete catalysis of 025% GTA) or 0.25% GTA for 5 days, with medium replenished every 48 hours. Growth dynamics were assessed by unbiased trypan blue exclusion based cytometry after 1, 3, and 5 days of treatment. A) In SCM, 36 mM NaAc plus 0.25% glycerol was more effective at growth reduction than GTA, but was also associated with decreased cell viability. B) In DM, GTA reduced growth of mesenchymal GBM12 GSCs greater than 36 mM NaAc plus 0.25% glycerol and did not reduce cell viability. n ≥ 3 independent experiments. *p < 0.05, **p ≤ 0.01, #p ≤ 0.001, ##p ≤ 0.0001, n.s.- not significant.