. Author manuscript; available in PMC: 2016 May 1.

Published in final edited form as: Biophys Chem. 2015 Mar 11;0:48–55. doi: 10.1016/j.bpc.2015.03.001

Table 2.

Comparison of ΔG°₃₇ values of H69 and mutants.

Name	pH 7.0		pH 5.5		Δ pH
Name	ΔG°₃₇ (kcal/mol)	ΔΔG°₃₇ (kcal/mol) (ΔG°_37ΨΨΨ − ΔG°_37UUU)	ΔG°₃₇ (kcal/mol)	ΔΔG°₃₇ (kcal/mol) (ΔG°_37ΨΨΨ − ΔG°_37UUU)	ΔΔG°₃₇ (kcal/mol) (ΔG°_{37pH 5.5} − ΔG°_{37pH 7.0})
UUU	−4.6 ± 0.1	−0.1 ± 0.2	−4.4 ± 0.1	−0.5 ± 0.2	0.2 ± 0.2
ΨΨΨ	−4.7 ± 0.1	−0.1 ± 0.2	−4.9 ± 0.1	−0.5 ± 0.2	−0.2 ± 0.2
UUU-A1912G	−4.7 ± 0.1	−0.2 ± 0.2	−4.7± 0.1	−0.5 ± 0.2	0.0 ± 0.2
ΨΨΨ-A1912G	−4.9 ± 0.1	−0.2 ± 0.2	−5.2 ± 0.1	−0.5 ± 0.2	−0.3 ± 0.2
UUC-U1917C	−4.4 ± 0.1	−0.3 ± 0.2	−4.4 ± 0.1	−0.5 ± 0.2	0.0 ± 0.2
ΨΨC-Ψ1917C	−4.7 ± 0.1	−0.3 ± 0.2	−4.9 ± 0.1	−0.5 ± 0.2	−0.2 ± 0.2
UUU-A1919G	−4.3 ± 0.1	−0.4 ± 0.2	−4.0 ± 0.1	−0.9 ± 0.2	0.3 ± 0.2
ΨΨΨ-A1919G	−4.7 ± 0.1	−0.4 ± 0.2	−4.9 ± 0.1	−0.9 ± 0.2	−0.2 ± 0.2

The thermodynamic effects of Ψ modifications and pH were determined as the free energy difference (ΔΔG°37).