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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Nat Methods. 2015 Apr 6;12(5):423–425. doi: 10.1038/nmeth.3351

Figure 2.

Figure 2

Removal of sequencing artifacts by Duplex Sequencing. (a) Exons in Abl spanning the active site of the enzyme were enriched by the double-capture protocol and sequenced conventionally on an Illumina HiSeq 2500. Despite extremely stringent quality filtering (minimum Phred score 50), and removal of end-repair artifacts by 5-nucleotide trimming from read ends, true mutations cannot be discerned among the thousands of sequencing errors that persist. (b) Duplex Sequencing of the same sample reveals a single point mutation in Abl which confers imatinib resistance. The mutation was verified by RT-PCR and Sanger sequencing.