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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Eur J Immunol. 2015 Jan 21;45(4):999–1009. doi: 10.1002/eji.201444625

Figure 6.

Figure 6

Trafficking of activated TDLN B cells in tumor-bearing and healthy mice. Activated 4T1 TDLN B cells were labeled with CMTMR and adoptively transferred into hosts with 4T1 intramammary fat pad tumor, or healthy hosts. (A) Phase contrast and fluorescence microscopy of CMTMR-stained B cells are shown. Original magnification × 200; white bar indicates 5 µm. (B) Percentages of transferred CMTMR-labelled TDLN B cells in TDLNs, spleen, lung and tumor in vivo. Labeled B cells were detected by flow cytometry. (C) Comparison of detected labeled B cells in lungs of tumor-bearing mice and healthy mice. Results are expressed as the mean percentages ± SEM of CD19+ labeled B cells (n= 5 mice). Results are shown from a single experiment representative of two experiments performed.