Figure 1.

Human mesenchymal stem cells (a-i), Adipogenic and osteogenic differentiation; (g,h) morphology and growth of fibroblastoid-adherent cells or placenta-derived Mesenchymal stem cells at phase 0 on days 2, 5 and 12, respectively (a-c). (a) Small, round cells (Magnification: ×100). (b,c) Long, thin cells (Magnification: ×100) with spindle-shape, which were reached to high confluence and were formed in a colony, as seen in the picture. (c) With morphology and growth of adipose-derived Mesenchymal stem cells at passage 0 on days 2, 4 and 8 (colony), respectively (d-f). (d) A single long spindle-shaped ADSC with thin processes; (Magnification: ×1000). (e,f) ADSCs that were growing (cells were created in a colony (f)) (Magnification: ×100). Adipogenic differentiation was evidenced by the formation of lipid vacuoles (yellow) by Oil Red O staining at passage three in adipose-derived MSC (g) (Magnification: ×1000). Osteogenic differentiation was evidenced by the formation of a mineralized matrix at passage three in adipose-derived MSC (Magnification: ×400) (h) Undifferentiated adipose MSC (i) Scale bar is 50 μm d, g, h) and 100 μm (a, b, c, e, f, i)