Figure 1.

Combined deletion of Ezh1 and Ezh2 resulted in the loss of H3K27me3 marks in hepatocytes. A) Representative photographs depict the mRNA expression of Ezh1 and Ezh2 by RNA-seq in WT, E1KO, E2KO, and E1/2KO livers at 3 mo of age. E1/2KO livers showed the lack of Ezh1 and Ezh2 expression. Exons encoding the SET domain of Ezh2 (red rectangle). B) Top panels are representative images of H3K27me3 immunofluorescence at 3 mo of age. H3K27me3 marks are absent in E1/2KO hepatocytes (arrows), but present in nonparenchymal cells (arrowheads). Bottom panels show gross liver morphology in mice of indicated genotypes at 8 mo of age. Only E1/2KO livers showed a nodular appearance (arrows). β-cat, β-catenin. C) Serum levels of ALT, AST, ALP, and albumin at 8 mo of age. Data are the mean ± sd (n = 5). *P < 0.05 as compared to the age-matched WT, E1KO, and E2KO.