Figure 5.

Liver damage and activation of HPCs during CCl₄-induced hepatotoxicity in E1/2KO mice. A) Up-regulation of cell cycle inhibitors and cell death-related genes in E1/2KO livers as determined by RNA-seq after CCl₄ injection. Cuffdiff (14) was used to detect significantly up-regulated genes with an FDR-adjusted P value of 0.05 (asterisk). B) Immunofluorescence staining for A6, a biliary/progenitor cell marker. Representative confocal images show the accumulation of A6-positive cells in portal and periportal areas of E1/2KO livers. Nuclei were counterstained with DAPI. C) Up-regulation of HPC-related genes in E1/2KO livers after the fourth injection of CCl₄ as determined by RNA-seq. Cuffdiff (14) was used to detect significantly up-regulated genes with an FDR-adjusted P value of 0.05 (asterisk).