Figure 4.

Brain-derived proteasomes from EAE-SJL mice generate MBP peptides recognized by MS-associated MHC class I molecules. A) Methodology of quantitative comparative analysis of peptides resulting from the hydrolysis of MBP by cerebral proteasomes from BALB/c mice in comparison with proteasomes from naive SJL and SJL mice developing EAE. MBP hydrolysis by cerebral proteasomes from BALB/c and SJL mice was performed in H₂¹⁸O and H₂¹⁶O, respectively, in order to quantitatively compare products of proteasome-mediated MBP degradation. B) Representative MS spectra (left) and quantitative estimation of the ratio of MHC class I–associated peptides in MBP hydrolysates (right) are shown. Data are shown as means ± sem (n = 3); **P < 0.01.