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Release of ENPVVHFF peptide is dramatically increased upon hydrolysis of MBP by cerebral proteasomes from EAE-SJL mice compared to nonimmunized SJL and BALB/c mice. Mass spectra (A), plotted time course (B) and 24-hour end point (C) of ENPVVHFF peptide release during MBP degradation by proteasomes isolated from control and autoimmune mice at the indicated time points monitored by an isotopically labeled internal standard. Data are presented as means ± sem (n = 2); **P < 0.01.
