Figure 4.
Pharmacologic inhibition of Gs-coupled 5-HTRs lowered superoxide formation in 661W cells incubated for 4 days in 30 mM glucose, whereas a cAMP analog (db cAMP, dibutyryl cAMP), a phosphodiesterase inhibitor (IBMX), or a PKA inhibitor (KT5720) increased it. SQ, SQ 22536, an adenylate cyclase inhibitor; LY, LY 215840, a 5-HT2R/5-HT7R antagonist; RO, RO 04-6790, a 5-HT6R antagonist; RS, RS 23597-190, a 5-HT4R antagonist. Studies were performed as described in the legend for Fig. 2. n ≥ 3 for all groups.