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Chromatogram for separation of catalpol from the partially purified crude sample of R. glutinosa by HSCCC. Solvent system: ethyl acetate–n-butanol–water (2:1:3, v/v/v); stationary phase: upper organic phase; mobile phase: lower aqueous phase; flow-rate: 1.0 mL min−1; revolution speed: 800 rpm; sample: 105 mg dissolved in 10 mL mixture solution of lower phase and upper phase (1:1, v/v) of the solvent system and retention of the stationary phase: 55%.
