Figure 3.

C-LOH on chromosome 4 validated by sequence analysis. (A) Enlargement of heat map from Figure 2, box indicates SNP gnf04.123.367 used for Sanger sequence analysis. Only samples shown in B and C are numbered; arrows point to tumors. (B) Sequencing chromatograms focusing on nucleotide polymorphism and shown for three tumors and three normal tissues from (BALB/c-neuT × FVB) F1 mice. Lines represent allelic measurements (A, solid line—BALB allele; C, dashed line—FVB allele). (C) Allelic ratios from genotype array for gnf04.123.367, represented as z-scores for normal and tumor DNA samples (negative z-score represents loss of BALB allele). Arrows indicate samples whose sequence chromatograms are shown in B.