Figure 4.

MiR-888 expression localizes to the cancerous epithelium. Serial tissue sections were cut from the EA38 EA tissue (A, C, and D) and the CS114 CS tissue (B, E, and F; see Table S1 for tissue sample details). These tissue samples were chosen for their high expression of miR-888 demonstrated by quantitative RT-PCR. For comparison, H&E-stained sections are shown in A and B. MiRNA in situ hybridization was performed using a probe complimentary to miR-888 (C and E) or a scrambled negative control probe (D and F). For C, the border between the cancerous epithelium and adjacent normal stromal is designated by a dotted line. For B and E, the border between the carcinomatous and sarcomatous elements is designated by a dotted line. In A and C, endometrial glands are designated by an asterisk (*).