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. Author manuscript; available in PMC: 2015 Apr 30.
Published in final edited form as: Methods Enzymol. 2011;491:3–24. doi: 10.1016/B978-0-12-385928-0.00001-8

Figure 1.6.

Figure 1.6

CFTR detection by Western blot. Representative results of CFTR protein detection in whole cell lysates by Western blot. Total cell lysates (15 μg) were separated by 8% PAGE, Western transferred and CFTR was detected using MM13-4 anti-CFTR monoclonal antibody, anti-mouse IgG-HRP (Pierce), and ECL (Pierce). Samples were analyzed in duplicates. Densitometry (Scion Image) was performed to assess CFTR expression levels in control and ER-stressed Calu-3 cells (immature CFTR, the core glycosylated ER form of CFTR; mature CFTR, the fully glycosylated, post-ER form of CFTR).