Fig. 3. FRCs are retained in the peritoneum.

(A) Mice received 350 μg of LPS 4 hours before intraperitoneal injection of 1 × 10⁶ allogeneic FRCs. Mice then received 4.5 mg of luciferin intraperitoneally and were imaged every 5 min for 45 min or until peak luminescence was reached. The maximum luminescence for each mouse over the imaging period was then recorded. The same maximum and minimum acquisition settings were used for all time points. Images depict FRC luminescence and localization in anesthetized recipient mice over time. Two mice most closely representing the group average are depicted (n = 5 mice per group). (B) Maximum luminescence over the imaging period was recorded for each mouse at each time point. Bars represent mean ± SEM (n = 3 mice per experiment). (C and D) Mice received 350 μg of LPS 4 hours before intraperitoneal injection of 1 × 10⁶ allogeneic antibody-labeled FRCs or saline (no stroma control). Blood (C) and spleen (D) were harvested after 1 h, 8 h, or 24 h. Donor FRCs were identified using flow cytometry. n = 2 (no stroma), n = 3 (1 h time point), n = 3 (8 h time point), n = 3 (24 h time point). Bars represent mean ± SEM.