Table 2.
Published Methods for Cell Seeding a Whole Liver ECM Scaffold.
| Author | ECM scaffold source species | Cell types | Number of cells | Method of cell delivery | Culture duration | Implantation site | Duration of graft survival |
|---|---|---|---|---|---|---|---|
| Baptista et al14 | Ferret | Human fetal liver cells Human umbilical vein endothelial cells |
7.0 × 10ˆ7 3.0 × 10ˆ7 |
Portal vein perfusion | 7 days | n/a | n/a |
| Bao et al10 | Rat | Rat Hepatocytes | 1.0 × 10ˆ8 | Portal vein perfusion | 6 h | In series with native liver via end-to-end anastomosis with the portal vein | 72 h |
| Barakat et al6 | Porcine | Human fetal stem cells Human fetal liver cells |
3.5 × 10ˆ8 1.0 × 10ˆ9 |
Portal and hepatic vein perfusion | 3, 7, 13 days | Into the infrahepatic space by using the recipient portal vein and infrahepatic inferior vena cava as an inflow and outflow, respectively | 2 h |
| Soto-Gutierrez et al7 | Rat | Mouse hepatocytes | 5.0 × 10ˆ7 | Injection or portal vein perfusion | 7 days | n/a | n/a |
| Uygun et al9 | Rat | Rat hepatocytes | 5.0 × 10ˆ7 | Portal vein perfusion | 14 days | Heterotopic, following unilateral nephrectomy | 8 h |