Figure 4.

E2 and STX reduce core body temperature (Tc) of ovariectomized female guinea pigs. Similar to E2 benzoate (EB)-treated females (8 μg/kg), STX (6 mg/kg) significantly decreased Tc compared with the propylene glycol vehicle (PPG). One week after ovariectomy, females were given daily sc injections of ligands at 10 am on M, W, and F. The data are presented as the mean ± SEM for each hour of the day averaged over 3 weeks of temperature probe recordings (ie, 3 weeks of temperature probe [SubCue DataLogger] measurements were collapsed down to a 24-h period). The data were analyzed using a two-way ANOVA (P < .05, F = 4.787, df = 2) with post hoc Newman-Keuls multiple comparison tests. All data points for both STX and EB were significantly different from vehicle (P < .01) except for the STX 2 pm to 4 pm hour time points (P < .05). The solid bar above the x-axis represents lights off or nighttime. (Reproduced from Roepke et al [168].)