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. 2015 Mar 20;29(5):730–738. doi: 10.1210/me.2014-1365

Figure 1.

Figure 1.

Expression of HDACs during a time course of osteoclastogenesis. Bone marrow cells from WT mice were cultured in 40-ng/mL MCSF during the first 3 days (d1–d3) to stimulate osteoclast precursor proliferation and then cultured in 40-ng/mL MCSF and 100-ng/mL RANKL for the last 3 days (d4–d6) to induce osteoclast differentiation. A–D, Expression of each member in the classic HDAC classes was quantified on each day by RT-QPCR (n = 3). A, Class I. B, Class IIa. C, Class IIb. D, Class IV. E, Expression of a representative osteoclast differentiation marker (n = 3). * and n.s. compare expression on d4, d5, or d6 (after RANKL treatment) with the expression on d3 (before RANKL treatment). Error bars, SD.