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Outline of NSPC culture system. Cerebral cortices from E14.5 mice are dissociated into single cells and cultured in the presence of EGF and FGF-1 to form neurospheres (85). After the dissociation of neurospheres, continued growth in EGF and FGF-1 will produce secondary neurospheres, whereas plating in the absence of EGF and FGF-1 can lead to differentiation.
