Table 3.
Detection of T. gondii DNA from bioassay inocula (homogenised tissues) compared to detection of T. gondii DNA from mouse brain (bioassay) from vaccinated and oocyst challenged pigs (G2)
| Pig No. | Brain | Other | Food | ||||||
|---|---|---|---|---|---|---|---|---|---|
| qPCR | ITS1 | Bio | qPCR | ITS1 | Bio | qPCR | ITS1 | Bio | |
| 820 | − | − | − | − | − | − | − | − | − |
| 821 | − | − | − | − | − | − | − | − | − |
| 822 | − | − | − | − | − | − | − | − | − |
| 823 | − | − | − | − | − | − | − | − | − |
| 824 | − | − | − | − | − | − | − | − | − |
| Positives | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 |
Results from the 529 bp T. gondii qPCR (qPCR), conventional T. gondii ITS1 PCR (ITS1) and ITS1 PCR from mouse brain – bioassay result (Bio), from vaccinated and oocyst challenged pigs (G2).