Table 4.
Detection of T. gondii DNA from bioassay inocula (homogenised tissues) compared to detection of T. gondii DNA from mouse brain (bioassay) from oocyst challenged pigs (G1)
| Pig No. | Brain | Other | Food | ||||||
|---|---|---|---|---|---|---|---|---|---|
| qPCR | ITS1 | Bio | qPCR | ITS1 | Bio | qPCR | ITS1 | Bio | |
| 825 | + | + | + | − | − | − | − | − | − |
| 826 | + | + | + | + | − | + | − | − | − |
| 827 | + | + | + | − | − | + | − | − | − |
| 828 | + | + | + | − | + | + | − | − | − |
| 829 | − | − | − | − | + | + | + | + | + |
| Positives | 4/5 | 4/5 | 4/5 | 1/5 | 2/5 | 4/5 | 1/5 | 1/5 | 1/5 |
Results from the 529 bp T. gondii qPCR (qPCR), conventional T. gondii ITS1 PCR (ITS1) and ITS1 PCR from mouse brain – bioassay result (Bio), from oocyst challenged pigs (G1).