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. 2015 Apr 30;4:6. doi: 10.1186/s13630-015-0015-z

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© Inaba; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Phylogenetic analysis of homologs of Ca ²⁺ sensor proteins in Excavata. Proteins (EF-hand proteins, length less than 350 amino acids) were searched against genomes of each excavate by BLASTP and those with E-value <e⁻⁹ were aligned with Ciona or Chlamydomonas Ca²⁺-sensors by CLUSTALW. An unrooted tree was drawn by MEGA5. Branches of each Ca²⁺-sensor are highlighted by colors. The protein sequences (with accession numbers indicated) were obtained from DDBJ/EMBL/GenBank, or from the genome browsers shown in the legend of Figure 5.