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. 2015 May 1;46(1):46. doi: 10.1186/s13567-015-0179-y

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© Maddison et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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PrP ^Sc characteristics of the scrapie isolates. Nine ovine hindbrain samples that were pooled and incubated with soil (a) were analysed by western blot. Murine isolates (b) designated G₃₃₈ (lanes 1, 2 and 3), Apl₃₃₈ii (lane 4), Apl₃₃₈ii/G₃₃₈ mixed phenotype (lane 5), Apl₃₃₈ii/P₃₃₈ mixed phenotype (Lane 6) and Cag₃₃₈ (Lane 7) are also shown. Blots were probed with anti-PrP antibody SHa31 and molecular mass markers of 20, 30 and 40 kDa are indicated. All samples were analysed twice to determine the molecular mass of unglycosylated PrP^Sc and gave consistent results. The strains G₃₃₈ (c) and Apl₃₃₈ii (d) were further analysed by the conformational stability assay and gave distinct profiles. These molecular traits were consistent both before (closed symbols) and after (open symbols) treatment with SDS and Napta. Analysis was carried out on 3 murine isolates of G₃₃₈ and 2 murine isolates of Apl₃₃₈ii and the presented data is representative of these isolates.