Table 1.
Genotypes and phenotypes of in vitro mutants and clinical isolates with fabI promoter mutations
| Strain | Background | sa-fabI promoter** | sa - fabI ‡ | sh - fabI ‡ | MIC* ‡ | MBC* ‡ | Comment |
|---|---|---|---|---|---|---|---|
| RN4220 | - | wt | wt | - | 1 | 2 | |
| MW2 | - | wt | wt | - | 0.12 | 0.12 | |
| ATCC6538 | - | wt | wt | - | 0.12 | 0.25 | |
| MO036 | RN4220 | A7G | mutated | - | 4 | 8 | |
| MO035 | RN4220 | C34T | mutated | - | 8 | 8 | |
| MO047 | RN4220 | C34T | mutated | - | 4 | 8 | |
| MO049 | RN4220 | C34T | mutated | - | 4 | 8 | |
| MO076 | MW2 | C34T | mutated | - | 4 | 8 | |
| CR002 | ATCC6538 | C34T | mutated | - | 4 | 8 | |
| MO034 | RN4220 | A72G | mutated | - | 8 | 8 | |
| MO077 | MW2 | A101- | mutated | - | 4 | 32 | 1 bp deletion |
| d7 | ATCC6538 | A101- | mutated | - | 2 | 8 | 1 bp deletion |
| MO051 | ATCC6538 | T109G | mutated | - | 4 | 8 | |
| MO052 | ATCC6538 | T109G | mutated | - | 8 | 16 | |
| MO053 | ATCC6538 | T109G | mutated | - | 4 | 8 | |
| MO055 | ATCC6538 | T109G | mutated | - | 4 | 8 | |
| QBR-102278-1097 | - | C34T | mutated | - | 0.25 | 32 | |
| QBR-102278-1889 | - | C34T | wt | - | 0.5 | 16 | |
| QBR-102278-1969 | - | C34T | wt | - | 0.25 | 32 | |
| QBR-102278-2095 | - | C34T | wt | - | 0.25 | 32 | |
| QBR-102278-2546 | - | C34T | mutated | + | 1 | 64 | |
| QBR-102278-2363 | - | A101C | wt | + | 16 | 32 | |
| QBR-102278-1052 | - | T109G | wt | + | 0.5 | 64 | IS256 insertion |
*MIC and MBC to triclosan are expressed as mg/L. **Polymorphic sites are indicated counting backwards from the sa-fabI start site of S. aureus Mu50 (GenBank ID: BA000017; position 1060308). Strains analysed by microarray are indicated in bold. ‡With the exception of strain QBR-102278-2095, these data have been previously reported [22].