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. Author manuscript; available in PMC: 2015 Apr 30.
Published in final edited form as: Virology. 2005 Jun 5;336(2):184–197. doi: 10.1016/j.virol.2005.03.032

Fig. 2.

Fig. 2

The R3A Env shows enhanced sensitivity to sCD4 and reduced sensitivity to Leu3a. (A) R3A Env is sensitive to sCD4 relative to R3B Env. TZM-bl cells were infected with virus incubated with sCD4 for 2 h prior to infection. Infection was quantitated by luciferase assay 48 h post-infection and values were normalized to infection in the absence of sCD4. Shown is a representative of 3 experiments with error bars derived from duplicate samples. (B) R3A Env is resistant to Leu-3a relative to R3B Env. Sup-T1 cells were preincubated with the indicated dose of Leu-3a for 1 h prior to infection with pseudotyped NL4-luc. Infection achieved by spinoculation was quantitated by luciferase assay 48 h post-infection and values were normalized to infection in the absence of Leu-3a. Shown is a representative of 3 experiments with error bars derived from quadruplicate samples (*P <0.05 for R3B vs. either NL4-3 or R3A).