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. Author manuscript; available in PMC: 2015 Apr 30.
Published in final edited form as: Virology. 2005 Jun 5;336(2):184–197. doi: 10.1016/j.virol.2005.03.032

Fig. 4.

Fig. 4

The R3A Env causes cytopathicity through CXCR4-dependent fusion when expressed in T cells. (A) 1G5 cells were transduced with a retroviral vector expressing HIV Env. Env surface levels were detected 3 days post-transduction using the 2G12 monoclonal antibody or human IgG1 as an isotype control. Shown is a representative stain 3 days post-transduction. The percentage of cells in each quadrant is indicated. (B) R3A Env induces extensive syncytia formation. Syncytia were observed by light microscopy 3 days post-transduction. Shown are representative photos of Sup-T1 cells 3 days post-transduction. (C) The number of live cells surviving 3 days post-transduction was quantitated by trypan blue exclusion. Shown is a representative of 8 independent experiments for Sup-T1 cells. (D and E) R3A-induced cytopathicity is dependent on fusion through CXCR4. Vector or R3A-transduced Sup-T1 cells were incubated with no drug, with T20 (D), or with AMD-3100 (E), and cytopathicity was quantitated as in C. Shown is a representative of 2 independent experiments (*P <0.05 for R3A and R3B vs. vector transduced cells).