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. 2004 Jul 1;32(Web Server issue):W176–W180. doi: 10.1093/nar/gkh401

Figure 2.

Figure 2

Analysis of the synthetic CYB5 gene. About 10 µl of each sample are analyzed on a 1% agarose gel. Lanes: (L) 100 bp DNA ladder (NEB); (1) negative control, second PCR without template DNA; (2) LCR product before second PCR; (3) second PCR without primer after LCR; (4) second PCR after LCR; (5) assembly PCR product before second PCR; (6) second PCR without primer after assembly PCR; (7) second PCR after assembly PCR; and (8) positive control, second PCR using yeast genomic DNA as template. The arrow indicates the 388 bp CYB5 PCR product.