Table 1.
One hundred and seventeen proteins identified or predicted to be in the outer membrane of the Arabidopsis chloroplast envelope.a
| AGI no.b | Name | Referencesc | Enveloped | MitoOMe | |
|---|---|---|---|---|---|
| SOLUTE/ION TRANSPORT | |||||
| At1g20816 | OEP21-1 | (i)(ii)(iii) | YES | ||
| At1g45170 | OEP24-1 | (i)(ii)(iii)(iv) | |||
| At1g76405 | OEP21-2 | (i)(ii)(iv) | YES | ||
| At2g01320 | WBC7 | (ii)(iii)(iv) | YES | ||
| At2g17695 | OEP23/DUF1990 | (vii) | YES | ||
| At2g28900 | OEP16-1 | (i)(ii)(iii)(iv) | YES | ||
| At2g43950 | OEP37 | (i)(ii)(iii)(iv) | YES | ||
| At3g51870 | PAPST1 homolog | (viii) | YES | ||
| At3g62880 | OEP16-4 | (i)(ii) | |||
| At4g16160 | OEP16-2 | (i)(ii) | |||
| At5g42960 | OEP24-2 | (i)(ii) | YES | ||
| PROTEIN IMPORT COMPONENTS AND THEIR HOMOLOGS | |||||
| At1g02280 | Toc33 | (i)(ii) | YES | ||
| At2g16640 | Toc132 | (i)(ii)(iii)(iv) | YES | ||
| At2g17390 | AKR2B | (iii) | |||
| At3g16620 | Toc120 | (i)(ii)(iii)(iv) | |||
| At3g17970 | Toc64-III | (i)(ii)(iii)(iv) | YES | ||
| At3g44160 | P39/OEP80tr1 | (ii) | |||
| At3g46740 | Toc75-III | (i)(ii)(iii)(iv) | YES | ||
| At3g48620 | P36/OEP80tr2 | (ii) | |||
| At4g02510 | Toc159 | (i)(ii)(iii)(iv) | YES | ||
| At4g09080 | Toc75-IV | (i)(ii) | |||
| At5g05000 | Toc34 | (i)(ii)(iii)(iv) | YES | ||
| At5g19620 | OEP80/Toc75-V | (i)(ii)(iv) | YES | ||
| At5g20300 | Toc90 | (i)(ii)(iv) | |||
| PROTEIN TURNOVER AND MODIFICATION | |||||
| At1g02560 | ClpP5 (proteolysis) | (iv) | YES | ||
| At1g07930 | E-Tu (protein synthesis) | (iii) | |||
| At1g09340 | HIP1.3/Rap38/CSP41B (protein synthesis) | (iv) | YES | ||
| At1g63900 | SP1 (proteolysis) | (vi) | |||
| At1g67690 | M3 protease | (iv) | |||
| At3g46780 | pTAC16 (transcription) | (iv) | YES | ||
| At4g05050 | UBQ11 (proteolysis) | (iii)(iv) | |||
| At4g32250 | Tyrosine kinase | (iii)(iv) | YES | ||
| At4g36650 | pBrP (transcription) | (ix) | |||
| At5g16870 | PTH2 family (protein synthesis) | (iii)(iv) | (x) | ||
| At5g35210 | PTM (transcription) | (ii) | YES | ||
| At5g56730 | peptidase M16 family | (iv) | YES | (xi) | |
| LIPID METABOLISM | |||||
| At1g77590 | LACS9 | (i)(ii)(iii)(iv) | YES | ||
| At2g11810 | MGD3 | (i)(ii) | |||
| At2g27490 | ATCOAE | (iii)(iv) | YES | ||
| At2g38670 | PECT1 | (iv) | (x) | ||
| At3g06510 | SFR2/GGGT | (ii)(iii)(iv) | YES | ||
| At3g06960 | TGD4 | (ii) | YES | ||
| At3g11670 | DGD1 | (i)(ii) | |||
| At3g26070 | PAP/FBN3a | (iv) | YES | ||
| At3g63170 | FAP1 | (iii) | YES | ||
| At4g00550 | DGD2 | (i)(ii) | |||
| At4g15440 | HPL homolg | (i)(ii) | YES | ||
| At5g20410 | MGD2 | (i)(ii) | |||
| CARBOHYDRATE METABOLISM AND REGULATION | |||||
| At1g12230 | transaldolase | (iv) | YES | ||
| At1g13900 | PAP2 | (v) | (x)(xi) | ||
| At2g19860 | HXK2 | (iv) | (x) | ||
| At4g29130 | HXK1 | (iii)(iv) | YES | (x) | |
| OTHER METABOLISM AND REGULATION | |||||
| At1g34430 | PDC E2 | (iv) | YES | ||
| At1g44170 | ALDH3H1 | (iv) | |||
| At2g34590 | PDC E1beta | (iv) | YES | ||
| At2g47770 | TSPO | (ii) | |||
| At3g01500 | beta CA1 | (iv) | YES | ||
| At3g16950 | PDC E3 | (iv) | YES | ||
| At3g25860 | PDC E2 | (iv) | YES | ||
| At3g27820 | MDAR4 | (iii)(iv) | YES | ||
| At5g17770 | CBR | (iii)(iv) | (x) | ||
| At5g23190 | CYP86B1 | (i) | |||
| At5g25900 | KO1/GA3 | (ii) | |||
| INTRACELLULAR COMMUNICATION | |||||
| At2g16070 | PDV2 (division) | (i)(ii)(iii) | YES | ||
| At2g20890 | THF1/PSB29 (plasma membrane) | (i) | YES | ||
| At3g25690 | CHUP1 (actin-dependent movement)) | (ii) | YES | ||
| At5g53280 | PDV1 (division) | (i)(ii) | |||
| At5g58140 | PHOT2 (actin-dependent movement) | (iii)(iv) | YES | ||
| FUNCTIONS/LOCATIONS DEFINED IN COMPARTMENTS | |||||
| OTHER THAN THE CHLOROPLAST OUTER ENVELOPE | |||||
| At1g27390 | Tom20-2 (mito) | (iii) | (x)(xi) | ||
| At3g01280 | VDAC1 (mito) | (i) | YES | (x) | |
| At3g12580 | Hsc70-4 (cytosol) | (iv) | |||
| At3g21865 | PEX22 (peroxisome) | (iv) | |||
| At3g46030 | histone H2B (nucleus) | (iii) | |||
| At3g63150 | MIRO2 (mito) | (iv) | (x)(xi) | ||
| At4g14430 | enoyl-CoA isomerase (peroxisome) | (iii) | |||
| At4g16450 | Complex I subunit (mito) | (iii) | |||
| At4g31780 | MGD1 (IEM) | (iii) | YES | ||
| At4g35000 | APX3 (peroxisome) | (iii)(iv) | YES | (xi) | |
| At4g38920 | vacuolar ATPase sub | (iii) | |||
| At5g02500 | HSC70-1 (cytosol/nucleus) | (iv) | YES | ||
| At5g06290 | Prx B (stroma) | (iv) | YES | ||
| At5g15090 | VDAC3 (mito) | (i) | YES | (x) | |
| At5g27540 | EMB2473/MIRO1 (mito) | (iv) | (x)(xi) | ||
| At5g35360 | CAC2/BC (IEM) | (iv) | YES | ||
| FUNCTIONS UNKNOWN/UNCLEAR | |||||
| At1g09920 | (iii) | (xi) | |||
| At1g16000 | OEP9 | (ii) | |||
| At1g27300 | (iii) | ||||
| At1g64850 | (iv) | YES | |||
| At1g68680 | (iii) | YES | |||
| At1g70480 | DUF220 | (iii)(iv) | |||
| At1g80890 | OEP9.2 | (ii) | |||
| At2g06010 | (iv) | ||||
| At2g24440 | (iii) | ||||
| At2g32240 | DUF869 | (iii)(iv) | (xi) | ||
| At2g32650 | PTAC18 like | (iv) | |||
| At2g44640 | (iii) | YES | |||
| At3g26740 | CCL | (iii) | |||
| At3g49350 | (iii) | ||||
| At3g52230 | OMP24 homolog | (i)(ii)(iii) | YES | ||
| At3g52420 | OEP7 | (i)(ii) | |||
| At3g53560 | TPR protein | (iii) | YES | ||
| At3g63160 | OEP6 | (ii) | YES | ||
| At4g02482 | putative GTPase | (ii) | |||
| At4g15810 | NTPase | (ii) | |||
| At4g17170 | RAB2 | (iv) | YES | ||
| At4g27680 | NTPase | (iii)(iv) | |||
| At4g27990 | YGGT-B protein | (iii) | YES | ||
| At5g11560 | (iv) | ||||
| At5g20520 | WAV2 | (iv) | (x) | ||
| At5g21920 | YGGT-A | (iii) | |||
| At5g21990 | OEP61-TPR | (ii) | |||
| At5g27330 | (iii) | ||||
| At5g42070 | (iv) | YES | |||
| At5g43070 | WPP1 | (iii) | |||
| At5g51020 | CRL | (ii)(iii)(iv) | YES | ||
| At5g59840 | RAB8A-like | (iv) | |||
| At5g64816 | (iii) | YES | |||
Names and functional categories are based on literatures cited in this work and databases. See Supplementary Material Table S1 for the extended name (if any), the location curated by various databases, and other predicted properties based on the primary sequence for each protein.
Arabidopsis gene identifier (AGI) number, which represents the systematic designation given to each locus, gene, and its corresponding protein product by The Arabidopsis Information Resource (TAIR: https://www.arabidopsis.org/).
This list includes in total 117 proteins from two earlier review articles [32 from (i) Inoue (2007) and 44 from (ii) Breuers et al. (2011)], two recent chloroplast outer envelope proteomics studies [50 from (iii) Simm et al. (2013) and 58 from (iv) Gutierrez-Carbonell et al. (2014),] and five reports on individual outer envelope proteins [(v) PAP2 by Sun et al. (2012), (vi) SP1 by Ling et al. (2012), (vii) OEP23 by Goetze et al. (2015), (viii) PAPST1 by Xu et al. (2013), and (ix) pBrP by Lagrange et al. (2003)]. Note that Gigolashvili et al. (2012) predicts inner-envelope localization of PAPST1, and that the AGI number for pBrP was updated from At4g36655.
YES indicates that the given protein was found in the chloroplast envelope proteomic studies (Ferro et al., 2003, 2010; Froehlich et al., 2003), which are listed in The Plant Proteome Database (PPDB: http://ppdb.tc.cornell.edu/) (Sun et al., 2009).