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. 2015 Apr 30;6:304. doi: 10.3389/fpls.2015.00304

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Copyright © 2015 Zhou, Sun, Zhao, Pan and Lu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Diurnal expression of the luciferase gene (LUC) driven by the 1400 bp upstream fragment of the QH6 promoter, with the G-box intacted (1400::LUC) or mutated (1400M::LUC). Arabidopsis thaliana Col-0 plants were transformed by 1400::LUC and 1400M::LUC constructs. The activity of luciferase expressed was quantified by measuring the radiance emitted and captured in 4 min by IVIS Lumina XR Imaging System (Caliper Life Sciences). Plants were growing in standard 12h/12h light/dark conditions. ZT is Zeitgeber time and here represents time from light on at dawn. Filled and open bars indicate subjective days and nights. Data represent means ± SD. The differences of expression levels were assessed by unpaired Student's t-test. ^*p < 0.05 and ^**p < 0.01 in 1400::LUC vs. 1400M::LUC transgenic Col-0 plants.