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. 2015 Apr 30;6:382. doi: 10.3389/fmicb.2015.00382

FIGURE 5.

FIGURE 5

PrkA changes the expression of sigK via negatively regulating the transcription factor of Hpr (ScoC). (A) The sequence of the sigK promoter regions by 5′ deletions. The rectangle boxes indicate the binding motifs in the promoter of sigK including SigE, Hpr(ScoC), GlnR, and SigD. (B) Mapping of the sigK promoter regions by 5′ deletions. Schematic representation of the different sigK::lacZ fusions used in this study. The filled arrows indicate the primers used for generating the various reporter fusions (P1–P5). The blocks also indicate the binding motifs in the promoter of sigK including SigE, Hpr(ScoC), GlnR, and SigD, whereas the 5′ and 3′ end termini of the motifs are denoted with their nucleotide position relative to the initiator codon. The derivative strains of WT B. subtilis 168 or ΔprkA mutant carry the respective fusions are shown the below. (C) Expression of the series of sigK::lacZ fusions, respectively. Cells were grown in LB medium at 37°C, and β-galactosidase activities were determined at 36 h. The left histograms represent the derivative strains of the WT background; the right histograms represent the derivative strains of the ΔprkA mutant background. ∗P < 0.01; n.s represented no statistical significance.