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. 2015 Apr 16;2015:816019. doi: 10.1155/2015/816019

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Copyright © 2015 Justyna Karolczak et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Myosin VI is a target of PKA. (a) A putative PKA phosphorylation site (threonine residue 1104 in the mouse MVI heavy chain, in green) is located in the vicinity of a very conserved region within the RRL partner recognition cluster (in red). In blue, the putative PKC phosphorylation sites. The kinase specific phosphorylation sites were depicted with a NetPhosK 1.0 Server (http://www.cbs.dtu.dk/services/NetPhosK/). The MVI sequences used for analyses are human (Q9UM54), rat (D4A5I9), and mouse (Q64331). (b) In vitro PKA phosphorylation assay. Left panel: Coomassie brilliant blue stained gel (CBB-staining), right panel: ³²P autoradiogram. Lane 1: the catalytic kinase domain; lane 2: GST; lane 3: GST-tagged MVI globular tail domain. ∗ Points to the ~52-kDa band of interest (GST-MVI domain).