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Binding ability of 2A9 and 2D7 to EpCAM was detected on Colo205 and HHCC cells by flow cytometry. Cells were incubated with biotin labeled EpCAM antibody (2A9 or 2D7, red histograms) or isotype control (black histograms) antibody (20 μg/mL) for 40 min at 4°C. Then cells were washed and stained with FITC-labeled secondary antibody and analyzed by flow cytometry.
