Figure 1.

Identifying conserved protein interaction pathways with PathBLAST. PathBLAST operates in two modes, depending on whether the query is a single pathway or a whole network. In the first mode, single user-defined pathways are queried against a reference network of observed protein interactions from bacteria, yeast, fly or worm. In the second mode, two large protein networks are aligned against each other to enumerate all of the pathways that are conserved between them. High-scoring pathway matches (A–D) are ranked by score and indicate pathways that are potentially conserved over evolution. The current focus of the PathBLAST website is on the first (more common) mode of query.