| 5.0 ul | 10× T4 RNA ligase Buffer |
| 5.0 ul | 10 mM ATP |
| 5.0 ul | DMSO |
| 5.8 ul | Glycerol (RNA grade) |
| 10.0 ul | [5’-32P] pCp (made in steps 1 and 2 above) |
| 2.0 ul | T4 RNA ligase |
| 17.2 ul | RNA with DEPC-DDW to make a total volume of 50 ul |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.