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. Author manuscript; available in PMC: 2015 Apr 30.
Published in final edited form as: Methods Enzymol. 2014 Dec 26;551:387–403. doi: 10.1016/bs.mie.2014.10.021
40.0 ul labeling mixture (from section 3 and 4 above, save the other 10 ul for step 7)
12.0 ul 5 M NaCl
2.0 ul 0.5 M EDTA
2.0 ul RNase A/T1 mixture
1.0 ul yeast tRNA (10 mg/ml)
143.0 ul DEPC-DDW to make a total volume of 200 ul