| 4 ul | 5×1st strand buffer |
| 1ul | RNasin (20 U/ul) |
| 2 ul | 0.1 M DTT |
| 1ul | 10 mM dNTP |
| 1ul | 10 mM ATP |
| 1ul | T4 DNA ligase (10 U) |
| 3 ul | DEPC-DDW |
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