Analysis of Circadian Regulation of Poly(A) Tail Length

. Author manuscript; available in PMC: 2015 Apr 30.

Published in final edited form as: Methods Enzymol. 2014 Dec 26;551:387–403. doi: 10.1016/bs.mie.2014.10.021

2.0 ul	10× PCR buffer
0.8 ul	50 mM MgCl₂
0.4 ul	10 mM dNTP
0.4 ul	Oligo(dT)anchor (25 pmol/ul)
0.4 ul	Message-specific primer (25 pmol/ul)
0.2 ul	Taq polymerase
1.6 ul	PAT cDNA (original or diluted)
14.2 ul	DDW to make a total volume of 20 ul