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Effects of wash modifiers on removal of HCPs associated with different mAbs. HCP levels were assessed by ELISA after wash with phosphate buffer (PBS), 50 mM sodium caprylate (Cap), 0.5 M tetramethylammonium chloride (TMAC), 1% (w/v) CHAPS, 1 M sodium chloride (NaCl), 0.5 M arginine-HCl (Arg), and 1 M urea. All wash modifiers were prepared in phosphate buffer at pH 7
