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. 2014 Jul 26;30(5):1114–1124. doi: 10.1002/btpr.1948

Table 3.

Comparison of the Levels of HCPs That Remained Associated With mAb1-Sepharose After Wash With One or Combination of Two Modifiers

Inline graphic

The levels of HCPs are expressed as percentage of levels observed for PBS control. Inline graphic 0-20 %, Inline graphic 21-40 %, Inline graphic 41-60 %, Inline graphic 61-80 %, Inline graphic 81-100 %

NaCl: 1 M sodium chloride; urea: 1 M urea; cap: 50 mM sodium caprylate; arg: 0.5 M arginine–HCl. The same concentrations were used for combination of two wash modifiers as for single modifiers. All wash modifiers were prepared in phosphate buffer at pH 7.