Figure 3. Expression of a pET30a-WOX2 fusion protein. Lane 1, uninduced pET30a-WOX2; lanes 2–3, pET30a-WOX2 induced by 0.5 mM IPTG for 3 and 5 h, respectively; lane 4, uninduced pET30a; lane 5, induced pET30a for 3 h; lane 6, negative control E. coli BL21 (DE3); M, Protein Molecular Weight Marker (Low) (TaKaRa). The target protein is indicated with arrows.