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. 2015 Apr 30;5:9783. doi: 10.1038/srep09783

Figure 1. ASS1 silencing in MKN45 and 3IB2 cell clones decreased ASS1 protein expression but did not affect cell proliferation in vitro.

Figure 1

(a) ASS1 protein expression was determined in human MKN45 cells and ASS1 shRNA stable transfectants. (b) The proliferation of MKN45 cells and ASS1 shRNA stable transfectants was determined at 24, 48, and 72 h. (c) Ass1 expression was determined in murine 3IB2 cells and ASS1 shRNA stable transfectants. (d) The proliferation of 3IB2 cells and ASS1 shRNA stable transfectants was determined at 24, 48, and 72 h. The results of Western blot analysis of protein expression, which were obtained from three independent experiments, are shown in Supplementary Figure S2. The bars represent the mean ± s.d. P: parental cells; VC: vector control; RNAi-1 and RNAi-2: ASS1-specific shRNAs 1 and 2, respectively. NS, not significant, *P < 0.01, **P < 0.001, ***P < 0.0001.