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. 2015 Apr 30;5:9783. doi: 10.1038/srep09783

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ASS1 knockdown cell clones were grown in culture medium containing 5% FCS. Two different medium conditions were used. Arg (-) indicates arginine-free medium. Cells were cultured for 24, 48, or 72 h. (a&b) Cell proliferation. (c&d) Wound healing. P: parental cells; VC: vector control; RNAi-1 and RNAi-2: ASS1-specific shRNAs 1 and 2, respectively. The results are expressed as the mean ± s.d. of three independent experiments. NS, not significant, **P < 0.001, ***P < 0.0001.